MSH6 Antibody

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NSJ Bioreagents
Product Code: NSJ-V7592
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7592-20UG20 ug£264.00
Quantity:
NSJ-V7592-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: MST6-2
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the MSH6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human colon carcinoma with MSH6 antibody (clone MST6-2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
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Immunofluorescence staining of PFA-fixed human MCF-7 cells with MSH6 antibody (green, clone MST6-2) and Phalloidin (red).
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Flow cytometry testing of fixed and permeabilized human MCF-7 cells with MSH6 antibody (clone MST6-2); Red=isotype control, Blue= Estrogen Receptor alpha antibody.
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IHC staining of FFPE human colon carcinoma with MSH6 antibody (clone MST6-2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Immunofluorescence staining of PFA-fixed human MCF-7 cells with MSH6 antibody (green, clone MST6-2) and Phalloidin (red).
Flow cytometry testing of fixed and permeabilized human MCF-7 cells with MSH6 antibody (clone MST6-2); Red=isotype control, Blue= Estrogen Receptor alpha antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Optimal dilution of the MSH6 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
MSH6 is a component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant human MSH6 protein fragment within amino acids 374-540 was used as the immunogen for the MSH6 antibody.
Limitation:
This MSH6 antibody is available for research use only.
Localization:
Nucleus
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P52701