MUC1 Antibody / Mucin-1

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Titration of the recombinant MUC1 may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

This MAb reacts with the DTRP epitope in the tandem repeats. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA/Mucin-1/MUC1 may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions.In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to MUC1 is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

4582

Full length human protein was used as the immunogen for this recombinant MUC1 antibody.

This recombinant MUC1 antibody is available for research use only.

Cytoplasmic and cell surface

Protein A affinity chromatography

Human

P15941