Nucleophosmin Antibody / NPM1

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,

Optimal dilution of the Nucleophosmin antibody should be determined by the researcher.

Recognizes a 33kDa glycoprotein, identified as Nucleophosmin (NPM). It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

A GST fusion protein containing the N-terminal portion of Nucleophosmin fused to a 14 amino acid portion of ALK protein was used as the immunogen for the Nucleophosmin antibody.

This Nucleophosmin antibody is available for research use only.

Nuclear, cytoplasmic

Yes

Protein G affinity chromatography

Human

P06748