Nucleoli Marker Antibody

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NSJ Bioreagents
Product Code: NSJ-V7035
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7035-20UG20 ug£264.00
Quantity:
NSJ-V7035-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: NCM102-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Nucleoli Marker antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of Nucleoli Marker antibody and FFPE human tonsil tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.~
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Immunofluorescent staining of AF488 conjugated Nucleoli Marker
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IHC testing of Nucleoli Marker antibody and FFPE human tonsil tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.~
Immunofluorescent staining of AF488 conjugated Nucleoli Marker

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Titering of the Nucleoli Marker antibody may be required for optimal performance.

2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
The nucleolus is the largest structure in the nucleus of eukaryotic cells, where it primarily serves as the site of ribosome synthesis and assembly. Nucleoli also have other important functions like assembly of signal recognition particles and playing a role in the cell's response to stress. Nucleoli are made of proteins and RNA and form around specific chromosomal regions. [Wiki]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
Unknown
Immunogen:
A preparation of myeloid leukemia cell nuclei was used as the immunogen.
Limitation:
This Nucleoli Marker antibody is available for research use only.
Localization:
Nucleoli
Purity:
Protein G affinity chromatography
Species Reactivity :
Human