Neurofilament Antibody, phospho (NF-H)

Western blot: 1-2ug/ml,Flow cytometry: 0.5-1ug/million cells,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT

Optimal dilution of the NF-H antibody (phospho) should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

This mAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Crude neurofilament preparation from porcine spinal cord was used as the immunogen for the NF-H antibody (phospho).

This NF-H antibody (phospho) is available for research use only.

Cytoplasmic

Protein G affinity chromatography

Human, Mouse, Rat

P12036