Nestin Antibody

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NSJ Bioreagents
Product Code: NSJ-V7665
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7665-20UG20 ug£264.00
Quantity:
NSJ-V7665-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG
Antibody Clonality: Monoclonal
Antibody Clone: NES/2911
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Nestin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 5
IHC staining of FFPE human kidney with Nestin antibody (clone NES/2911). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human kidney with Nestin antibody (clone NES/2911). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human colon carcinoma with Nestin antibody (clone NES/2911). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
4 / 5
IHC staining of FFPE human colon carcinoma with Nestin antibody (clone NES/2911). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
5 / 5
SDS-PAGE analysis of purified, BSA-free Nestin antibody (clone NES/2911) as confirmation of integrity and purity.
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IHC staining of FFPE human kidney with Nestin antibody (clone NES/2911). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human kidney with Nestin antibody (clone NES/2911). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma with Nestin antibody (clone NES/2911). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma with Nestin antibody (clone NES/2911). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Nestin antibody (clone NES/2911) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Nestin is a major intermediate filament (IF) protein of embryonic central nervous system progenitor cells. It is also a component of the dynamic IF network during muscle development, where it polymerizes with Desmin and Vimentin. Nestin co-assembles with Vimentin or -internexin and forms heterodimer coiled-coil molecules which then further assemble into 10 nml IFs. Deletion of the IF consensus rod domain in nestin alters nestin localization in CNS precursor cells and radial glial cells in vivo. Nestin is a marker for neuroepithelial stem cells, glioma cells and tumor endothelial cells during rapid growth. During axon elongation of differentiation neurons, nestin localizes to the growth cones and may play a role in growth cone guidance. In the rat adrenal gland, nestin is expressed by the zona fasciculata and the zona reticularis. Nestin is also expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant human partial protein (amino acids 698-844) was used as the immunogen for the Nestin antibody.
Limitation:
This Nestin antibody is available for research use only.
Localization:
Cytoplasm, membrane
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P48681