CD73 Antibody

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NSJ Bioreagents
Product Code: NSJ-V7570
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7570-20UG20 ug£264.00
Quantity:
NSJ-V7570-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: NT5E/2503
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the CD73 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human tonsil tissue with CD73 antibody (clone NT5E/2503). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
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IHC staining of FFPE human lung carcinoma with CD73 antibody (clone NT5E/2503). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
3 / 8
IHC staining of FFPE human colon carcinoma with CD73 antibody (clone NT5E/2503). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
4 / 8
Immunofluorescence staining of human U-87 MG cells with CD73 antibody (green, clone NT5E/2503) and Reddot nuclear stain (red).
5 / 8
Flow cytometry testing of human U-87 MG cells with CD73 antibody (clone NT5E/2503); Red=isotype control, Blue= CD73 antibody.
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Western blot testing of human 1) A431 and 2) PC-3 cell lysate with CD73 antibody (clone NT5E/2503). Predicted molecular weight: 60-70 kDa.
7 / 8
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD73 antibody (clone NT5E/2503). These results demonstrate the foremost specificity of the NT5E/2503 mAb.<br>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
8 / 8
SDS-PAGE analysis of purified, BSA-free CD73 antibody (clone NT5E/2503) as confirmation of integrity and purity.
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IHC staining of FFPE human tonsil tissue with CD73 antibody (clone NT5E/2503). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human lung carcinoma with CD73 antibody (clone NT5E/2503). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma with CD73 antibody (clone NT5E/2503). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Immunofluorescence staining of human U-87 MG cells with CD73 antibody (green, clone NT5E/2503) and Reddot nuclear stain (red).
Flow cytometry testing of human U-87 MG cells with CD73 antibody (clone NT5E/2503); Red=isotype control, Blue= CD73 antibody.
Western blot testing of human 1) A431 and 2) PC-3 cell lysate with CD73 antibody (clone NT5E/2503). Predicted molecular weight: 60-70 kDa.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD73 antibody (clone NT5E/2503). These results demonstrate the foremost specificity of the NT5E/2503 mAb.<br>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free CD73 antibody (clone NT5E/2503) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the CD73 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A recombinant full length human NT5E protein was used as the immunogen for the CD73 antibody.
Limitation:
This CD73 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P21589