CD73 Antibody

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NSJ Bioreagents
Product Code: NSJ-V7588BTN
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7588BTN-500ul500 ul£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: NT5E/2646
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the CD73 antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).

Images

1 / 4
IHC staining of FFPE human tonsil tissue with biotinylated CD73 antibody (clone NT5E/2646). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human tonsil tissue with biotinylated CD73 antibody (clone NT5E/2646). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
3 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD73 antibody (clone NT5E/2646). These results demonstrate the foremost specificity of the NT5E/2646 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
4 / 4
SDS-PAGE analysis of purified, BSA-free CD73 antibody (clone NT5E/2646) as confirmation of integrity and purity.
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IHC staining of FFPE human tonsil tissue with biotinylated CD73 antibody (clone NT5E/2646). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with biotinylated CD73 antibody (clone NT5E/2646). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD73 antibody (clone NT5E/2646). These results demonstrate the foremost specificity of the NT5E/2646 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free CD73 antibody (clone NT5E/2646) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
ELISA: order Ab without BSA for coating,Flow cytometry: 2-4ug/million cells in 0.1ml,Immunofluorescence: 2-4ug/ml,Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the CD73 antibody should be determined by the researcher.
Description:
CD73 is a membrane-bound extracellular enzyme overexpressed in several cancer types. Its expression has been linked to poor prognosis in melanoma, colorectal, gastric, triple negative breast cancer, and to a pro-metastatic phenotype in prostate cancer. Together with CD39, it plays a major role in promoting immunosuppression through the pathway degrading adenosine triphosphate (ATP) into adenosine. Within the tumor microenvironment, ATP promotes immune cell-mediated killing of cancer cells. In contrast, adenosine accumulation causes immune suppression, dysregulation of immune cell infiltrates and stimulates angiogenesis resulting in tumor spreading. CD73 is active on the last step of the degradation pathway, where it is the enzyme that actually degrades AMP into adenosine. CD73-blockade promotes anti-tumor immunity by reducing adenosine accumulation. Accordingly, anti-CD73 mAbs stimulate anti-tumor immunity and reduce tumor metastasis in mouse tumor models, and could enhance the efficacy of treatment with anti-PD1 or anti-CTLA4 antibodies.
Format :
Biotin Conjugate
Formulation :
0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human NT5E protein was used as the immunogen for the CD73 antibody.
Limitation:
This CD73 antibody is available for research use only.
Localization:
Cell surface
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P21589