KRT7 Antibody / Cytokeratin 7

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NSJ Bioreagents
Product Code: NSJ-V2654BTN
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2654BTN-500ul500 ul£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: OV-TL12/30
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Cytokeratin 7 antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).

Images

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IHC staining of FFPE human prostate carcinoma with Cytokeratin 7 antibody (clone OV-TL12/30). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human prostate carcinoma with Cytokeratin 7 antibody (clone OV-TL12/30). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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SDS-PAGE analysis of purified, BSA-free Cytokeratin 7 antibody (clone OV-TL12/30) as confirmation of integrity and purity.
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IHC staining of FFPE human prostate carcinoma with Cytokeratin 7 antibody (clone OV-TL12/30). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate carcinoma with Cytokeratin 7 antibody (clone OV-TL12/30). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Cytokeratin 7 antibody (clone OV-TL12/30) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Flow cytometry: 5ul per test per one million cells or 5ul per 100ul of whole blood,Immunofluorescence: 2-4ug/ml,Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml at RT
Application Note:
Optimal dilution of the Cytokeratin 7 antibody should be determined by the researcher.
Description:
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
Format :
Biotin Conjugate
Formulation :
0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
The ovarian carcinoma cell line OTN 11 was used as the immunogen for the Cytokeratin 7 antibody.
Limitation:
This Cytokeratin 7 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P08729