PCNA Antibody

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NSJ Bioreagents
Product Code: NSJ-V7998
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7998-20UG20 ug£264.00
Quantity:
NSJ-V7998-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: PC5
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human tonsil with PCNA antibody (clone PC5). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human colon carcinoma with PCNA antibody (clone PC5). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
Western blot testing of human samples with PCNA antibody (clone PC5). Expected molecular weight: 29-36 kDa.
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IHC staining of FFPE human tonsil with PCNA antibody (clone PC5). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma with PCNA antibody (clone PC5). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human samples with PCNA antibody (clone PC5). Expected molecular weight: 29-36 kDa.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A rat PCNA/protein A fusion protein was used at the immunogen for this PCNA antibody.
Limitation:
This PCNA antibody is available for research use only.
Localization:
Predominantly nuclear, some cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P12004