Thymidine Phosphorylase Antibody / PD-ECGF

Western blot: 0.5-1ug/ml,Immunoprecipitation: 0.5-1ug/500ug protein lysate,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Thymidine Phosphorylase antibody to be titered up or down for optimal performance.

Recognizes a 485 amino acid protein (55 kDa monomer / 110 kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), also called Thymidine Phosphorylase (TP, Tymp) or Gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5 -deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Human recombinant full length protein was used as the immunogen for this Thymidine Phosphorylase antibody.

This Thymidine Phosphorylase antibody is available for research use only.

Cytoplasmic, nuclear

Protein G affinity chromatography

Human, Mouse, Rat

P19971