CDK1 Antibody
| Code | Size | Price |
|---|
| NSJ-V2083-20UG | 20 ug | £264.00 |
Quantity:
| NSJ-V2083-100UG | 100 ug | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: POH-1
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store the CDK1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Documents
Further Information
Application Details :
Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CDK1 antibody to be titered up or down for optimal performance.
1. Staining of FFPE tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Description:
This antibody recognizes a 34kDa protein, which is identified as cyclin dependent kinase 1 (Cdk1) or p34cdc2 protein kinase. CDK1 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated CDK1 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
983
Immunogen:
Purified recombinant human Cdk1 / p34cdc2 was used as the immunogen for this CDK1 antibody.
Limitation:
This CDK1 antibody is available for research use only.
Localization:
Nuclear and Cytoplasmic
Purity:
Protein G purified antibody
Species Reactivity :
Human
Uniprot #:
P06493