Progesterone Receptor Antibody

NSJ Bioreagents
Product Code: NSJ-V2226
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2226-20UG20 ug£264.00
Quantity:
NSJ-V2226-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: PR501
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Progesterone receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of normal human breast stained with Progesterone receptor antibody (PR501).
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IHC testing of invasive ductal carcinoma stained with progesterone receptor antibody (PR501).
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SDS-PAGE Analysis of Purified, BSA-Free Progesterone Receptor Antibody (clone PR501). Confirmation of Integrity and Purity of the Antibody.

IHC testing of normal human breast stained with Progesterone receptor antibody (PR501).
IHC testing of invasive ductal carcinoma stained with progesterone receptor antibody (PR501).
SDS-PAGE Analysis of Purified, BSA-Free Progesterone Receptor Antibody (clone PR501). Confirmation of Integrity and Purity of the Antibody.

Further Information

Application Details :
Western blot: 0.5-1ug/ml for 2 hr at RT,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Description:
This antibody is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa). Expression of progesterone receptor has been suggested to reflect a intact estrogen regulatory machinery and therefore predict better clinical response to endocrine therapy than ER alone.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
5241
Immunogen:
Recombinant human Progesterone Receptor protein was used as the immunogen for this antibody.
Limitation:
This Progesterone receptor antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human