CD45 Antibody (Leukocyte marker)

Immunohistology (FFPE): 0.5-1.0ug/ml for 30 min at RT

Due to variation in protocol and secondary antibody used, the CD45 antibody may need to be titered for optimal performance.

CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Human B lymphocytes were used as the immunogen for the CD45 antibody.

This CD45 antibody is available for research use only.

Cell surface and cytoplasmic

Protein G affinity chromatography

Human

P08575