Phosphotyrosine Antibody / P-Tyr

NSJ Bioreagents
Product Code: NSJ-V8317
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8317-20UG20 ug£264.00
Quantity:
NSJ-V8317-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: PY265
Regulatory Status: RUO
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store the Phosphotyrosine antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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SDS-PAGE analysis of purified, BSA-free Phosphotyrosine antibody (clone PY265) as confirmation of integrity and purity.

SDS-PAGE analysis of purified, BSA-free Phosphotyrosine antibody (clone PY265) as confirmation of integrity and purity.

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Phosphotyrosine was used as the immunogen for the Phosphotyrosine antibody.
Limitation:
This Phosphotyrosine antibody is available for research use only.
Purity:
Protein G affinity chromatography
Species Reactivity :
Phosphotyrosine