AKT1 Antibody
| Code | Size | Price |
|---|
| NSJ-V7745-20UG | 20 ug | £264.00 |
Quantity:
| NSJ-V7745-100UG | 100 ug | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rAKT1/2491
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store the recombinant AKT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Documents
Further Information
Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Amino acids 85-189 from the human protein were used as the immunogen for the recombinant AKT1 antibody.
Limitation:
This recombinant AKT1 antibody is available for research use only.
Localization:
Nucleus, plasma membrane, cytoplasm
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P31749