Retinoblastoma Antibody / Rb1

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Due to variation in protocol and secondary antibody used, the recombinant RB1 antibody may need to be titered for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

A human recombinant partial protein was used as immunogen for this recombinant RB1 antibody.

This recombinant RB1 antibody is available for research use only.

Nuclear

Protein A affinity chromatography

Human, Mouse

P06400