Glycophorin A Antibody

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NSJ Bioreagents
Product Code: NSJ-V3604
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3604-20UG20 ug£264.00
Quantity:
NSJ-V3604-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rGYPA/280
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant GYPA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human angiosarcoma with recombinant GYPA antibody (clone rGYPA/280). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.~
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Western blot testing of human K562 cell lysate with Glycophorin A
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IHC testing of FFPE human angiosarcoma with recombinant GYPA antibody (clone rGYPA/280). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.~
Western blot testing of human K562 cell lysate with Glycophorin A

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Application Note:
Optimal dilution of the recombinant GYPA antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant human Glycophorin A protein was used as the immunogen for the recombinant GYPA antibody.
Limitation:
This recombinant GYPA antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P02724