CD3 Antibody

NSJ Bioreagents
Product Code: NSJ-V2380
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2380-20UG20 ug£264.00
Quantity:
NSJ-V2380-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG3, kappa
Antibody Clonality: Monoclonal
Antibody Clone: RIV9
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Functional Study
  • Immunofluorescence (IF)
Storage:
CD3 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Images

1 / 3
Immunofluorescent staining of FFPE human Jurkat cells with CD3 antibody (green, clone RIV9) and Reddot nuclear stain (red).
2 / 3
Flow cytometry testing of human Jurkat cells with CD3 antibody (clone RIV9); Red=isotype control, Blue= CD3 antibody.
3 / 3
Flow cytometry testing of lymphocyte-gated human PBM cells with CF488A-CD3 antibody (clone RIV9); Gray=unstained, Green= CD3 antibody.

Immunofluorescent staining of FFPE human Jurkat cells with CD3 antibody (green, clone RIV9) and Reddot nuclear stain (red).
Flow cytometry testing of human Jurkat cells with CD3 antibody (clone RIV9); Red=isotype control, Blue= CD3 antibody.
Flow cytometry testing of lymphocyte-gated human PBM cells with CF488A-CD3 antibody (clone RIV9); Gray=unstained, Green= CD3 antibody.

Further Information

Application Details :
Functional studies (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the CD3 antibody for a specific application should be determined by researcher.
Description:
The CD3 complex mediates signal transduction. [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Human peripheral lymphocytes were used as immunogen for the CD3 antibody.
Limitation:
The CD3 antibody is available for research use only.
Localization:
Cell surface and cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P07766