PSA / Prostate Specific Antigen Antibody

NSJ Bioreagents
Product Code: NSJ-V8699
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8699-20UG20 ug£264.00
Quantity:
NSJ-V8699-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rKLK3/4753
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant Prostate Specific Antigen antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human prostate carcinoma with recombinant Prostate Specific Antigen antibody (clone rKLK3/4753). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
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SDS-PAGE analy

IHC staining of FFPE human prostate carcinoma with recombinant Prostate Specific Antigen antibody (clone rKLK3/4753). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
SDS-PAGE analy

Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the recombinant Prostate Specific Antigen antibody should be determined by the researcher.
Description:
Recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA). This MAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. This mAb makes an excellent pair with clone 1A7G6B6 for PSA tests.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human KLK3/PSA protein was used as the immunogen for the recombinant Prostate Specific Antigen antibody.
Limitation:
This recombinant Prostate Specific Antigen antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P07288