H3K14ac Antibody / Acetyl Histone H3 Lysine 14

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NSJ Bioreagents
Product Code: NSJ-R20199
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20199-25UG25ug£321.00
Quantity:
NSJ-R20199-100UG100ug£581.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM130
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • ChIP
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Western Blot (WB)
Storage:
Store the recombinant H3K14ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western Blot of acid extracts from human HeLa cells untreated (-) or treated (+) with sodium butyrate using the recombinant H3K14ac antibody at 0.5 ug/ml.
2 / 5
ICC testing of human HeLa cells treated with sodium butyrate using recombinant H3K14ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
3 / 5
ChIP performed on HeLa cells using the recombinant H3K14ac antibody (5ug). Real-time PCR was performed using primers specific to the gene indicated.
4 / 5
The recombinant H3K14ac antibody specifically reacts to Histone H3 acetylated at Lysine 14 (K14ac). No cross reactivity with acetylated Lysine 4 (K4ac), 9 (K9ac), 18 (K18ac), 23 (K23ac), 27 (K27ac), 36 (K36ac), or 79 (K79ac) in Histone H3.
5 / 5
The binding specificity of recombinant H3K14ac antibody to Histone H3 acetylated at Lysine 14 (K14ac) is not affected by the modification of neighboring amino acids.
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Western Blot of acid extracts from human HeLa cells untreated (-) or treated (+) with sodium butyrate using the recombinant H3K14ac antibody at 0.5 ug/ml.
ICC testing of human HeLa cells treated with sodium butyrate using recombinant H3K14ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
ChIP performed on HeLa cells using the recombinant H3K14ac antibody (5ug). Real-time PCR was performed using primers specific to the gene indicated.
The recombinant H3K14ac antibody specifically reacts to Histone H3 acetylated at Lysine 14 (K14ac). No cross reactivity with acetylated Lysine 4 (K4ac), 9 (K9ac), 18 (K18ac), 23 (K23ac), 27 (K27ac), 36 (K36ac), or 79 (K79ac) in Histone H3.
The binding specificity of recombinant H3K14ac antibody to Histone H3 acetylated at Lysine 14 (K14ac) is not affected by the modification of neighboring amino acids.

Documents

Further Information

Application Details :
Western blot: 0.5-2ug/ml,Immunocytochemistry: 0.5-2ug/ml,Chromatin IP (ChIP): 2-10ug/mg of lysate,ELISA: 0.2-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H3K14ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant H3K14ac antibody reacts to Histone H3 acetylated at Lysine 14 (K14ac), which is not affected by the modification of neighboring amino acids. No cross reactivity with acetylated Lysine 4 (K4ac), 9 (K9ac), 18 (K18ac), 23 (K23ac), 27 (K27ac), 36 (K36ac), or 79 (K79ac) in histone H3.
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys14) was used as the immunogen for this recombinant H3K14ac antibody.
Limitation:
This recombinant H3K14ac antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
All Species
Uniprot #:
P84243