H3K4me1 Antibody / Monomethyl Histone H3 Lysine 4

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NSJ Bioreagents
Product Code: NSJ-R20200
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20200-25UG25ug£321.00
Quantity:
NSJ-R20200-100UG100ug£581.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM140
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • ChIP
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Western Blot (WB)
Storage:
Store the recombinant H3K4me1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot of recombinant histone H3.3 (1) and acid extracts of human HeLa cells (2) using the recombinant H3K4me1 antibody at 0.5 ug/ml showed a band of histone H3 monomethylated at Lysine 4 (K4me1).
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ICC testing of human HeLa cells treated with sodium butyrate using recombinant H3K4me1 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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ChIP performed on human HeLa cells using 5ug recombinant H3K4me1 antibody. Real-time PCR was performed using primers specific to the gene indicated.
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Recombinant H3K4me1 antibody specifically reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated (K4me2), trimethylated (K4me3), or other methylations in histone H3.
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Western blot of recombinant histone H3.3 (1) and acid extracts of human HeLa cells (2) using the recombinant H3K4me1 antibody at 0.5 ug/ml showed a band of histone H3 monomethylated at Lysine 4 (K4me1).
ICC testing of human HeLa cells treated with sodium butyrate using recombinant H3K4me1 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
ChIP performed on human HeLa cells using 5ug recombinant H3K4me1 antibody. Real-time PCR was performed using primers specific to the gene indicated.
Recombinant H3K4me1 antibody specifically reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated (K4me2), trimethylated (K4me3), or other methylations in histone H3.

Documents

Further Information

Application Details :
Western blot: 0.2-1ug/ml,Chromatin IP (ChIP): 2-10ug/mg of lysate,Immunocytochemistry: 1-2ug/ml,ELISA: 0.2-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H3K4me1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant H3K4me1 antibody reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2) or trimethylated Lysine 4 (K4me3), or other methylations in histone H3
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys4) was used as the immunogen for this recombinant H3K4me1 antibody.
Limitation:
This recombinant H3K4me1 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
All Species
Uniprot #:
P84243