H3K36me3 Antibody / Trimethyl Histone H3 Lysine 36

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NSJ Bioreagents
Product Code: NSJ-R20204
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20204-25UG25ug£321.00
Quantity:
NSJ-R20204-100UG100ug£581.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM155
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • ChIP
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Store the recombinant H3K36me3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using the recombinant H3K36me3 antibody at 2 ug/ml showed a band of Histone H3 trimethylated at Lysine 36 (K36me3) in HeLa cells.
2 / 3
ChIP performed on HeLa cells using the recombinant H3K36me3 antibody (5ug). Real-time PCR was performed using primers specific to the gene indicated.
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The recombinant H3K36me3 antibody specifically reacts to Histone H3 trimethylated at Lysine 36 (K36me3). No cross reactivity with non-modified (K36 Ctrl), monomethylated (K36me1) or dimethylated Lysine 36 (K36me2), or other methylations in Histone H3.
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Western blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using the recombinant H3K36me3 antibody at 2 ug/ml showed a band of Histone H3 trimethylated at Lysine 36 (K36me3) in HeLa cells.
ChIP performed on HeLa cells using the recombinant H3K36me3 antibody (5ug). Real-time PCR was performed using primers specific to the gene indicated.
The recombinant H3K36me3 antibody specifically reacts to Histone H3 trimethylated at Lysine 36 (K36me3). No cross reactivity with non-modified (K36 Ctrl), monomethylated (K36me1) or dimethylated Lysine 36 (K36me2), or other methylations in Histone H3.

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Further Information

Application Details :
Western blot: 0.5-2ug/ml,Chromatin IP (ChIP): 2-10ug/mg of lysate,ELISA: 0.2-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H3K36me3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant H3K36me3 antibody reacts to Histone H3 trimethylated at Lysine 36 (K36me3). No cross reactivity with monomethylated Lysine 36 (K36me1) or dimethylated Lysine 36 (K36me2), or other methylations in histone H3.
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys36) was used as the immunogen for this recombinant H3K36me3 antibody.
Limitation:
This recombinant H3K36me3 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
All Species
Uniprot #:
P84243