Histone H3 Antibody (pThr3)

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NSJ Bioreagents
Product Code: NSJ-R20233
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20233-25UG25ug£321.00
Quantity:
NSJ-R20233-100UG100ug£581.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM159
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Western Blot (WB)
Storage:
Store the recombinant phospho-Histone H3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot test of acid extracts of HeLa cells non-treated (1) or treated (2) with Nocodazole, and recombinant Histone H3.3 (3), using recombinant phospho-Histone H3 antibody at 0.1 ug/ml, showed a band of Histone H3 phosphorylated at Threonine 3 in HeLa cells.
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ICC/IF of HeLa cells using recombinant phospho-Histone H3 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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ICC/IF of HeLa cells using recombinant phospho-Histone H3 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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Western blot test of acid extracts of HeLa cells non-treated (1) or treated (2) with Nocodazole, and recombinant Histone H3.3 (3), using recombinant phospho-Histone H3 antibody at 0.1 ug/ml, showed a band of Histone H3 phosphorylated at Threonine 3 in HeLa cells.
ICC/IF of HeLa cells using recombinant phospho-Histone H3 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
ICC/IF of HeLa cells using recombinant phospho-Histone H3 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Documents

Further Information

Application Details :
Western blot: 0.1-1ug/ml,Immunocytochemistry: 0.5-2ug/ml,ELISA: 0.2-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant phospho-Histone H3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant phospho-Histone H3 antibody reacts to Histone H3 phosphorylated at Threonine 3. No cross reactivity with other phosphorylated histones
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A phospho-peptide corresponding to phospho-Histone H3 (Thr3) was used as the immunogen for this recombinant phospho-Histone H3 antibody.
Limitation:
This recombinant phospho-Histone H3 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
All Species
Uniprot #:
P84243