H3K18ac Antibody / Acetyl Histone H3 Lysine 18

Contact us
NSJ Bioreagents
Product Code: NSJ-R20209
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20209-25UG25ug£321.00
Quantity:
NSJ-R20209-100UG100ug£581.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM166
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • ChIP
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant H3K18ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

1 / 6
Western blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate using recombinant H3K18ac antibody at 0.5 ug/mL showed a band of Histone H3 acetylated at Lysine 18 in treated HeLa cells.
2 / 6
Sandwich ELISA with acetylated Histone H3 at Lys 18 using human HeLa cell lysate, treated or untreated with sodium butyrate, using <a href=../search_result.php?search_txt=rm188>anti-Histone H3CT (RM188, 1 ug/ml)</a> as the capture and biotinylated recombinant H3K18ac antibody (RM166, 2 ug/ml) as the detect.
3 / 6
ChIP performed on HeLa cells with or without sodium butyrate treatment using recombinant H3K18ac antibody (5ug). Real-time PCR was performed using primers specific to the gene indicated.
4 / 6
The recombinant H3K18ac antibody specifically reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with acetylated 4 (K4ac), 9 (K9ac), 14 (K14ac), 23 (K23ac), 27 (K27ac), 36 (K36ac), 56 (K56ac), 79 (K79ac), or 122 (K122) in Histone H3.
5 / 6
ICC/IF test of HeLa cells treated with sodium butyrate using recombinant H3K18ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
6 / 6
ICC staining of human HepG2 cells using recombinant H3K18ac antibody.
❮❯

Western blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate using recombinant H3K18ac antibody at 0.5 ug/mL showed a band of Histone H3 acetylated at Lysine 18 in treated HeLa cells.
Sandwich ELISA with acetylated Histone H3 at Lys 18 using human HeLa cell lysate, treated or untreated with sodium butyrate, using <a href=../search_result.php?search_txt=rm188>anti-Histone H3CT (RM188, 1 ug/ml)</a> as the capture and biotinylated recombinant H3K18ac antibody (RM166, 2 ug/ml) as the detect.
ChIP performed on HeLa cells with or without sodium butyrate treatment using recombinant H3K18ac antibody (5ug). Real-time PCR was performed using primers specific to the gene indicated.
The recombinant H3K18ac antibody specifically reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with acetylated 4 (K4ac), 9 (K9ac), 14 (K14ac), 23 (K23ac), 27 (K27ac), 36 (K36ac), 56 (K56ac), 79 (K79ac), or 122 (K122) in Histone H3.
ICC/IF test of HeLa cells treated with sodium butyrate using recombinant H3K18ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
ICC staining of human HepG2 cells using recombinant H3K18ac antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-2ug/ml,Immunocytochemistry: 0.5-2ug/ml,Chromatin IP (ChIP): 2ug/ml-10ug/mg of lysate,Immunohistochemistry: 1-10ug/ml (1),ELISA: 0.2-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H3K18ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:
This recombinant H3K18ac antibody reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with other acetylated Lysines in histone H3.
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
An acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys18) was used as the immunogen for this recombinant H3K18ac antibody.
Limitation:
This recombinant H3K18ac antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
All Species
Uniprot #:
P84243