H3K18me2 Antibody / Dimethyl Histone H3 Lysine 18

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NSJ Bioreagents
Product Code: NSJ-R20217
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20217-25UG25ug£321.00
Quantity:
NSJ-R20217-100UG100ug£581.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM168
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Store the recombinant H3K18me2 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2) using recombinant H3K18me2 antibody at 0.5 ug/ml showed a band of Histone H3 dimethylated at Lysine 18 (K18me2) in HeLa cells.
2 / 3
A peptide dot blot shows the recombinant H3K18me2 antibody only reacts to Histone H3 dimethyl-Lysine 18 (K18me2). No cross reactivity with unmodified (K18Ctrl) or monomethylated Lysine 18 (K18me1), dimethylated Lysine 4 (K4me2) or dimethylated Lysine 36 (K36me2).
3 / 3
The recombinant H3K18me2 antibody specifically reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross reactivity with other methylated lysines in Histone H3.
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Western blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2) using recombinant H3K18me2 antibody at 0.5 ug/ml showed a band of Histone H3 dimethylated at Lysine 18 (K18me2) in HeLa cells.
A peptide dot blot shows the recombinant H3K18me2 antibody only reacts to Histone H3 dimethyl-Lysine 18 (K18me2). No cross reactivity with unmodified (K18Ctrl) or monomethylated Lysine 18 (K18me1), dimethylated Lysine 4 (K4me2) or dimethylated Lysine 36 (K36me2).
The recombinant H3K18me2 antibody specifically reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross reactivity with other methylated lysines in Histone H3.

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Further Information

Application Details :
Western blot: 0.2-1ug/ml,ELISA: 0.1ug/ml - 0.5ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H3K18me2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant H3K18me2 antibody reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross reactivity with monomethylated (K18me1) or trimethylated Lysine 18 (K18me3), or other methylation in Histone H3.
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys18) was used as the immunogen for this recombinant H3K18me2 antibody.
Limitation:
This recombinant H3K18me2 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
All Species
Uniprot #:
P84243