H3K23ac Antibody / Acetyl Histone H3 Lysine 23

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NSJ Bioreagents
Product Code: NSJ-R20229
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20229-25UG25ug£321.00
Quantity:
NSJ-R20229-100UG100ug£581.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM169
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Western Blot (WB)
Storage:
Store the recombinant H3K23ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot test of acid extracts of HeLa cells non-treated (1) or treated with sodium butyrate (2) and recombinant Histone H3.3 (3), using recombinant H3K23ac antibody at 1 ug/ml, showed a band of Histone H3 acetylated at Lysine 23 in treated HeLa cells.
2 / 3
This recombinant H3K23ac antibody specifically reacts to Histone H3 acetylated at Lysine 23 (K23ac). No cross reactivity with unmodified Lysine 23 (K23 ctrl) or other acetylated Lysines.
3 / 3
ICC/IF staining of HeLa cells treated with sodium butyrate using recombinant H3K23ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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Western blot test of acid extracts of HeLa cells non-treated (1) or treated with sodium butyrate (2) and recombinant Histone H3.3 (3), using recombinant H3K23ac antibody at 1 ug/ml, showed a band of Histone H3 acetylated at Lysine 23 in treated HeLa cells.
This recombinant H3K23ac antibody specifically reacts to Histone H3 acetylated at Lysine 23 (K23ac). No cross reactivity with unmodified Lysine 23 (K23 ctrl) or other acetylated Lysines.
ICC/IF staining of HeLa cells treated with sodium butyrate using recombinant H3K23ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Documents

Further Information

Application Details :
Western blot: 0.5-2ug/ml,ELISA: 0.5-1ug/ml,Immunocytochemistry: 1-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H3K23ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant H3K23ac antibody reacts to Histone H3 acetylated at Lysine 23 (K23ac). No cross reactivity with other acetylated Lysines in Histone H3.
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys23) was used as the immunogen for this recombinant H3K23ac antibody.
Limitation:
This recombinant H3K23ac antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
All Species
Uniprot #:
P84243