H3K27me3 Antibody / Trimethyl Histone H3 Lysine 27
| Code | Size | Price |
|---|
| NSJ-R20215-25UG | 25ug | £321.00 |
Quantity:
| NSJ-R20215-100UG | 100ug | £581.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM175
Regulatory Status: RUO
Target Species: Species Independent
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunocytochemistry (ICC)
- Western Blot (WB)
Storage:
Store the recombinant H3K27me3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Documents
Further Information
Application Details :
Western blot: 1-2ug/ml,Immunocytochemistry: 0.5-2ug/ml (1),ELISA: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H3K27me3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Description:
This recombinant H3K27me3 antibody reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity with monomethylated (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in Histone H3.
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys27) was used as the immunogen for this recombinant H3K27me3 antibody.
Limitation:
This recombinant H3K27me3 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
All Species
Uniprot #:
P84243