H3K79me2 Antibody / Dimethyl Histone H3 Lysine 79

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NSJ Bioreagents
Product Code: NSJ-R20220
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20220-25UG25ug£321.00
Quantity:
NSJ-R20220-100UG100ug£581.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM181
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • ChIP
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant H3K79me2 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2) using recombinant H3K79me2 antibody at 0.25 ug/ml showed a band of Histone H3 dimethylated at Lysine 79 (K79me2) in HeLa cells.
2 / 4
ChIP performed on HeLa cells using the recombinant H3K79me2 antibody (5ug). Real-time PCR was performed using primers specific to the gene indicated.
3 / 4
The recombinant H3K79me2 antibody specifically reacts to Histone H3 dimethylated at Lysine 79 (K79me2). Very slightly cross reactivity with monomethylated Lysine 14 (K14me1), and no cross reactivity with unmodified or trimethylated Lysine 79, or other H3 methylations.
4 / 4
ICC staining of human HepG2 cells with recombinant H3K79me2 antibody.
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Western blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2) using recombinant H3K79me2 antibody at 0.25 ug/ml showed a band of Histone H3 dimethylated at Lysine 79 (K79me2) in HeLa cells.
ChIP performed on HeLa cells using the recombinant H3K79me2 antibody (5ug). Real-time PCR was performed using primers specific to the gene indicated.
The recombinant H3K79me2 antibody specifically reacts to Histone H3 dimethylated at Lysine 79 (K79me2). Very slightly cross reactivity with monomethylated Lysine 14 (K14me1), and no cross reactivity with unmodified or trimethylated Lysine 79, or other H3 methylations.
ICC staining of human HepG2 cells with recombinant H3K79me2 antibody.

Documents

Further Information

Application Details :
Western blot: 0.25-1ug/ml,Immunohistochemistry: 0.1-1ug/ml (1),Chromatin IP (ChIP): 2-10ug/mg,ELISA: 0.2-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H3K79me2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:
This recombinant H3K79me2 antibody reacts to Histone H3 dimethylated at Lysine 79 (K79me2). No cross reactivity with monomethylated (K79me1) or trimethylated Lysine 79 (K79me3), or other methylation in Histone H3.
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys79) was used as the immunogen for this recombinant H3K79me2 antibody.
Limitation:
This recombinant H3K79me2 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
All Species
Uniprot #:
P84243