Histone H3 Antibody / Unmodified Lysine 4

NSJ Bioreagents
Product Code: NSJ-R20228
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20228-25UG25ug£321.00
Quantity:
NSJ-R20228-100UG100ug£581.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM186
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Western Blot (WB)
Storage:
Store the recombinant Histone H3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot test of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated and untreated with Nocodazole, and recombinant Histone H3.1 protein, using recombinant Histone H3 antibody at 1 ug/ml, showed a band of Histone H3 with unmodified Lys4.
2 / 3
ICC/IF staining of HeLa cells treated with sodium butyrate using recombinant Histone H3 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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This recombinant Histone H3 antibody specifically recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated, monomethylated, dimethylated, or trimethylated Lys4. The mAb binding specificity allows for modifications of Arg2 or Thr3 in Histone H3.

Western blot test of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated and untreated with Nocodazole, and recombinant Histone H3.1 protein, using recombinant Histone H3 antibody at 1 ug/ml, showed a band of Histone H3 with unmodified Lys4.
ICC/IF staining of HeLa cells treated with sodium butyrate using recombinant Histone H3 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
This recombinant Histone H3 antibody specifically recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated, monomethylated, dimethylated, or trimethylated Lys4. The mAb binding specificity allows for modifications of Arg2 or Thr3 in Histone H3.

Further Information

Application Details :
Western blot: 1-2ug/ml,ELISA: 0.5-1ug/ml,Immunocytochemistry: 0.5-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Histone H3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Histone H3 antibody recognizes Histone H3 that is unmodified at Lys4 but does not recognize acetylated or methylated Lys4. The recombinant Histone H3 antibody binding specificity allows for modifications of Arg2, Thr3, and/or other modifications in Histone H3.
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
An unmodified peptide corresponding to the N-terminus of Histone H3 was used as the immunogen for this recombinant Histone H3 antibody.
Limitation:
This recombinant Histone H3 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
All Species
Uniprot #:
Q16695