Histone H3 Antibody (Pan)

NSJ Bioreagents
Product Code: NSJ-R20252
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20252-25UG25ug£321.00
Quantity:
NSJ-R20252-100UG100ug£581.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM188
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Western Blot (WB)
Storage:
Store the recombinant Histone H3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

1 / 5
Western blot of recombinant Histone H3.3 and H3.1 proteins, A375, HEK293, HeLa and SK-MEL-2 whole cell lysates, using recombinant Histone H3 antibody at 0.025 ug/ml.
2 / 5
ICC/IF of HeLa cells using recombinant Histone H3 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
3 / 5
Sandwich ELISA against acetylated Histone H3 at Lys 9 using HeLa whole cell lysate, treated or untreated with sodium butyrate, using recombinant Histone H3 antibody (1ug/ml) as the capture and <a href=../search_result.php?search_txt=rm161>biotinylated anti-H3K9ac</a> (RM161, 1ug/ml) as the detect.
4 / 5
Sandwich ELISA against acetylated Histone H3 at Lys 79 using HeLa whole cell lysate, treated or untreated with sodium butyrate, using <a href=../tds/recombinant-h3k79ac-antibody-rabbit-monoclonal-acetyl-histone-h3-lysine-79-rm156-r20206>anti-H3K79ac</a> (RM156, 5 ug/ml) as the capture antibody and biotinylated recombinant Histone H3 antibody (RM188, 1 ug/ml) as the detect.
5 / 5
This recombinant Histone H3 antibody reacts to both Histone H3.1 and H3.3.

Western blot of recombinant Histone H3.3 and H3.1 proteins, A375, HEK293, HeLa and SK-MEL-2 whole cell lysates, using recombinant Histone H3 antibody at 0.025 ug/ml.
ICC/IF of HeLa cells using recombinant Histone H3 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Sandwich ELISA against acetylated Histone H3 at Lys 9 using HeLa whole cell lysate, treated or untreated with sodium butyrate, using recombinant Histone H3 antibody (1ug/ml) as the capture and <a href=../search_result.php?search_txt=rm161>biotinylated anti-H3K9ac</a> (RM161, 1ug/ml) as the detect.
Sandwich ELISA against acetylated Histone H3 at Lys 79 using HeLa whole cell lysate, treated or untreated with sodium butyrate, using <a href=../tds/recombinant-h3k79ac-antibody-rabbit-monoclonal-acetyl-histone-h3-lysine-79-rm156-r20206>anti-H3K79ac</a> (RM156, 5 ug/ml) as the capture antibody and biotinylated recombinant Histone H3 antibody (RM188, 1 ug/ml) as the detect.
This recombinant Histone H3 antibody reacts to both Histone H3.1 and H3.3.

Further Information

Application Details :
Western blot: 0.01-0.25ug/ml,Immunocytochemistry: 1ug/ml - 5ug/ml,ELISA: 0.2-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Histone H3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Histone H3 antibody reacts to all Histone H3 proteins, independent of post-translational modifications. No cross reactivity with other Histone proteins.
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the C-terminus of human Histone H3 was used as the immunogen for this recombinant Histone H3 antibody.
Limitation:
This recombinant Histone H3 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
All Species
Uniprot #:
P84243