Histone H4 Antibody (pSer1)

NSJ Bioreagents
Product Code: NSJ-R20222
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20222-25UG25ug£321.00
Quantity:
NSJ-R20222-100UG100ug£581.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM194
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Western Blot (WB)
Storage:
Store the recombinant phospho-Histone H4 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot of (1) acid extracts of HeLa cells and (2) recombinant Histone H4 using the recombinant phospho-Histone H4 antibody at 0.5 ug/ml showed a band of Histone H4 phosphorylated at Serine 1 in HeLa cells.
2 / 3
The recombinant phospho-Histone H4 antibody specifically reacts to Histone H4 phosphorylated at Ser1 (H4S1p). The reactivity is not affected by neighboring Arg3 modifications (H4S1pR3me1 and H4S1pR3me2). No cross reactivity with Histone H2A phosphorylated at Ser1 (H2AS1p).
3 / 3
ICC/IF of HeLa cells using recombinant phospho-Histone H4 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Western blot of (1) acid extracts of HeLa cells and (2) recombinant Histone H4 using the recombinant phospho-Histone H4 antibody at 0.5 ug/ml showed a band of Histone H4 phosphorylated at Serine 1 in HeLa cells.
The recombinant phospho-Histone H4 antibody specifically reacts to Histone H4 phosphorylated at Ser1 (H4S1p). The reactivity is not affected by neighboring Arg3 modifications (H4S1pR3me1 and H4S1pR3me2). No cross reactivity with Histone H2A phosphorylated at Ser1 (H2AS1p).
ICC/IF of HeLa cells using recombinant phospho-Histone H4 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Further Information

Application Details :
Western blot: 0.5-2ug/ml,Immunocytochemistry: 1-2ug/ml,ELISA: 0.2-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant phospho-Histone H4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant phospho-Histone H4 antibody reacts to Histone H4 phosphorylated at Serine 1. The reactivity is not affected by neighbor Arginine 3 modification. No cross reactivity with Histone H2A phosphorylated at Serine 1.
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A phospho-peptide corresponding to phospho-Histone H4 (pSer1) was used as the immunogen for this recombinant phospho-Histone H4 antibody.
Limitation:
This recombinant phospho-Histone H4 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
All Species
Uniprot #:
P62805