H4R3me1 Antibody / Monomethyl Histone H4 Arginine 3

Contact us
NSJ Bioreagents
Product Code: NSJ-R20223
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20223-25UG25ug£321.00
Quantity:
NSJ-R20223-100UG100ug£581.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM195
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Western Blot (WB)
Storage:
Store the recombinant H4R3me1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

1 / 3
Western blot test of (1) acid extracts of HeLa cells and (2) recombinant Histone H4 using recombinant H4R3me1 antibody at 0.5 ug/ml showed a band of Histone monomethylated at Arginine 3 in HeLa cells.
2 / 3
The recombinant H4R3me1 antibody specifically reacts to Histone H4 monomethylated at Arginine 3 (R3me1). No cross reactivity with unmodified Argi3 (R3 ctrl), asymmetric dimethylated Arg3 (R3me2a) or symmetric dimethylated Arg3 (R3me2s), and citrullinated Arg3 (R3Cit) in H4.
3 / 3
ICC/IF staining of HeLa cells treated with sodium butyrate using recombinant H4R3me1 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
❮❯

Western blot test of (1) acid extracts of HeLa cells and (2) recombinant Histone H4 using recombinant H4R3me1 antibody at 0.5 ug/ml showed a band of Histone monomethylated at Arginine 3 in HeLa cells.
The recombinant H4R3me1 antibody specifically reacts to Histone H4 monomethylated at Arginine 3 (R3me1). No cross reactivity with unmodified Argi3 (R3 ctrl), asymmetric dimethylated Arg3 (R3me2a) or symmetric dimethylated Arg3 (R3me2s), and citrullinated Arg3 (R3Cit) in H4.
ICC/IF staining of HeLa cells treated with sodium butyrate using recombinant H4R3me1 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Documents

Further Information

Application Details :
Western blot: 0.5-2ug/ml,ELISA: 0.2-1ug/ml,Immunocytochemistry: 1-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H4R3me1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant H4R3me1 antibody reacts to Histone H4 monomethylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arginine 3, asymmetric or symmetric dimethylated Arginine 3, and citrullinated Arginine 3 in Histone H4.
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A monomethyl-peptide corresponding to Monomethyl-Histone H4 (Arg3) was used as the immunogen for this recombinant H4R3me1 antibody.
Limitation:
This recombinant H4R3me1 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
All Species
Uniprot #:
P62805