H4K8ac Antibody / Acetyl Histone H4 Lysine 8

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NSJ Bioreagents
Product Code: NSJ-R20225
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20225-25UG25ug£321.00
Quantity:
NSJ-R20225-100UG100ug£581.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM201
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Western Blot (WB)
Storage:
Store the recombinant H4K8ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot test of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H4 using recombinant H4K8ac antibody at 0.5 ug/ml showed a band of Histone H4 acetylated at Lysine 8 in HeLa cells.
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This recombinant H4K8ac antibody specifically reacts to Histone H4 acetylated at Lysine 8 (K8ac). No cross reactivity with unmodified Lysine 8 (K8 ctrl) or other acetylated Lysines.
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ICC/IF staining of HeLa cells treated with sodium butyrate using recombinant H4K8ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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Western blot test of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H4 using recombinant H4K8ac antibody at 0.5 ug/ml showed a band of Histone H4 acetylated at Lysine 8 in HeLa cells.
This recombinant H4K8ac antibody specifically reacts to Histone H4 acetylated at Lysine 8 (K8ac). No cross reactivity with unmodified Lysine 8 (K8 ctrl) or other acetylated Lysines.
ICC/IF staining of HeLa cells treated with sodium butyrate using recombinant H4K8ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Documents

Further Information

Application Details :
Western blot: 0.5-2ug/ml,ELISA: 0.2-1ug/ml,Immunocytochemistry: 0.5-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H4K8ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant H4K8ac antibody reacts to Histone H4 acetylated at Lysine 8 (K8ac). No cross reactivity with other acetylated Lysines in Histone H4.
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
An acetyl-peptide corresponding to Acetyl-Histone H4 (Lys8) was used as the immunogen for this recombinant H4K8ac antibody.
Limitation:
This recombinant H4K8ac antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
All Species
Uniprot #:
P62805