Histone H4 Antibody

NSJ Bioreagents
Product Code: NSJ-R20254
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20254-25UG25ug£321.00
Quantity:
NSJ-R20254-100UG100ug£581.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM212
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Western Blot (WB)
Storage:
Store the recombinant Histone H4 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot of A375, HEK293, HeLa and SK-MEL-2 whole cell lysates using recombinant Histone H4 antibody at 0.2 ug/ml.
2 / 3
Western blot test of recombinant Histone H2A, H2B, H3.1, H3.3 and H4 proteins using recombinant Histone H4 antibody at 0.2 ug/ml.
3 / 3
ICC/IF of HeLa cells using recombinant Histone H4 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Western blot of A375, HEK293, HeLa and SK-MEL-2 whole cell lysates using recombinant Histone H4 antibody at 0.2 ug/ml.
Western blot test of recombinant Histone H2A, H2B, H3.1, H3.3 and H4 proteins using recombinant Histone H4 antibody at 0.2 ug/ml.
ICC/IF of HeLa cells using recombinant Histone H4 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunocytochemistry: 1-2ug/ml,ELISA: 0.2-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Histone H4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Histone H4 antibody reacts to the Histone H4 protein, independent of post-translational modifications. No cross reactivity with other histone proteins.
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the C-terminus of human Histone H4 was used as the immunogen for this recombinant Histone H4 antibody.
Limitation:
This recombinant Histone H4 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
All Species
Uniprot #:
P62805