Histone H2AX Antibody

NSJ Bioreagents
Product Code: NSJ-R20250
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20250-25UG25ug£321.00
Quantity:
NSJ-R20250-100UG100ug£581.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM214
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Western Blot (WB)
Storage:
Store the recombinant Histone H2AX antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot of A375, HEK293, HeLa and SK-MEL-2 whole cell lysates using recombinant Histone H2AX antibody at 0.5 ug/ml. Predicted molecular weight ~15 kDa.~
2 / 2
ICC/IF staining of human HeLa cells using recombinant Histone H2AX antibody (

Western blot of A375, HEK293, HeLa and SK-MEL-2 whole cell lysates using recombinant Histone H2AX antibody at 0.5 ug/ml. Predicted molecular weight ~15 kDa.~
ICC/IF staining of human HeLa cells using recombinant Histone H2AX antibody (

Further Information

Application Details :
Western blot: 0.5-2ug/ml,Immunocytochemistry: 1-2ug/ml,ELISA: 0.2-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Histone H2AX antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Histone H2AX antibody reacts to Histone H2AX protein, independent of post-translational modifications. No cross reactivity with other histone proteins.
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the C-terminus of human Histone H2AX was used as the immunogen for this recombinant Histone H2AX antibody.
Limitation:
This recombinant Histone H2AX antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
All Species
Uniprot #:
P16104