H2A.ZK4ac Antibody / Acetyl Histone H2A.Z Lysine 4

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NSJ Bioreagents
Product Code: NSJ-R20239
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20239-25UG25ug£321.00
Quantity:
NSJ-R20239-100UG100ug£581.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM221
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Western Blot (WB)
Storage:
Store the recombinant H2A.ZK4ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot test of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using recombinant H2A.ZK4ac antibody at 0.5 ug/ml, showed a band of Histone H2A.Z acetylated at Lysine 4 in treated HeLa cells.
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This recombinant H2A.ZK4ac antibody specifically reacts to Histone H2A.Z acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in Histone H2A.
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ICC/IF staining of HeLa cells treated with sodium butyrate using recombinant H2A.ZK4ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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Western blot test of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using recombinant H2A.ZK4ac antibody at 0.5 ug/ml, showed a band of Histone H2A.Z acetylated at Lysine 4 in treated HeLa cells.
This recombinant H2A.ZK4ac antibody specifically reacts to Histone H2A.Z acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in Histone H2A.
ICC/IF staining of HeLa cells treated with sodium butyrate using recombinant H2A.ZK4ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).

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Further Information

Application Details :
Western blot: 0.5-2ug/ml,ELISA: 0.2-1ug/ml,Immunocytochemistry: 1-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H2A.ZK4ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant H2A.ZK4ac antibody reacts to Histone H2A.Z acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H2A
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
An acetyl-peptide corresponding to Acetyl-Histone H2A.Z (Lys4) was used as the immunogen for this recombinant H2A.ZK4ac antibody.
Limitation:
This recombinant H2A.ZK4ac antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
All Species
Uniprot #:
P0C0S5