Phospho-Histone H2AX Antibody (pS139)

NSJ Bioreagents
Product Code: NSJ-R20244
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20244-25UG25ug£321.00
Quantity:
NSJ-R20244-100UG100ug£581.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM224
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Western Blot (WB)
Storage:
Store the recombinant Gamma H2AX antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot of acid extracts of HeLa cells treated or non-treated with UV, using recombinant Gamma H2AX antibody at 0.5 ug/ml, showed a band of Histone H2A.X phosphorylated at Serine 139 in HeLa cells.~
2 / 2
ICC/IF of HeLa cells using reco

Western blot of acid extracts of HeLa cells treated or non-treated with UV, using recombinant Gamma H2AX antibody at 0.5 ug/ml, showed a band of Histone H2A.X phosphorylated at Serine 139 in HeLa cells.~
ICC/IF of HeLa cells using reco

Further Information

Application Details :
Western blot: 0.5-2ug/ml,ELISA: 0.2-1ug/ml,Immunocytochemistry: 0.5-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Gamma H2AX antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Gamma H2AX antibody reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A phospho-peptide corresponding to phospho-Histone H2AX (S139) was used as the immunogen for this recombinant Gamma H2AX antibody.
Limitation:
This recombinant Gamma H2AX antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
All Species
Uniprot #:
P16104