H2BK20ac Antibody / Acetyl Histone H2B Lysine 20

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NSJ Bioreagents
Product Code: NSJ-R20241
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20241-25UG25ug£321.00
Quantity:
NSJ-R20241-100UG100ug£581.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM235
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Western Blot (WB)
Storage:
Store the recombinant H2BK20ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot test of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using recombinant H2BK20ac antibody at 0.5 ug/ml.
2 / 3
This recombinant H2BK20ac antibody specifically reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in Histone H2B.
3 / 3
ICC/IF staining of HeLa cells treated with sodium butyrate using recombinant H2BK20ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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Western blot test of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using recombinant H2BK20ac antibody at 0.5 ug/ml.
This recombinant H2BK20ac antibody specifically reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in Histone H2B.
ICC/IF staining of HeLa cells treated with sodium butyrate using recombinant H2BK20ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Documents

Further Information

Application Details :
Western blot: 0.5-2ug/ml,ELISA: 0.2-1ug/ml,Immunocytochemistry: 1-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H2BK20ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant H2BK20ac antibody reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
An acetyl-peptide corresponding to Acetyl-Histone H2B (Lys20) was used as the immunogen for this recombinant H2BK20ac antibody.
Limitation:
This recombinant H2BK20ac antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
Human
Uniprot #:
P33778