H2BK23ac Antibody / Acetyl Histone H2B Lysine 23

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NSJ Bioreagents
Product Code: NSJ-R20242
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20242-25UG25ug£321.00
Quantity:
NSJ-R20242-100UG100ug£581.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM260
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Western Blot (WB)
Storage:
Store the recombinant H2BK23ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot test of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H2B, using recombinant H2BK23ac antibody at 0.5 ug/ml.
2 / 3
This recombinant H2BK23ac antibody specifically reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with acetylated Lysine 5 (K5ac), 11 (K11ac), 12 (K12ac), 15 (K15ac), 20 (K20ac), or non-modified Lysine 23 in Histone H2B.
3 / 3
ICC/IF of HeLa cells treated with sodium butyrate, using recombinant H2BK23ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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Western blot test of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H2B, using recombinant H2BK23ac antibody at 0.5 ug/ml.
This recombinant H2BK23ac antibody specifically reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with acetylated Lysine 5 (K5ac), 11 (K11ac), 12 (K12ac), 15 (K15ac), 20 (K20ac), or non-modified Lysine 23 in Histone H2B.
ICC/IF of HeLa cells treated with sodium butyrate, using recombinant H2BK23ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Documents

Further Information

Application Details :
Western blot: 0.5-2ug/ml,Immunocytochemistry: 0.5-2ug/ml,ELISA: 0.2-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H2BK23ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant H2BK23ac antibody reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with other acetylated Lysines in histones.
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
An acetyl-peptide corresponding to Acetyl-Histone H2B (Lys23) was used as the immunogen for this recombinant H2BK23ac antibody.
Limitation:
This recombinant H2BK23ac antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
Human
Uniprot #:
P33778