BRAF V600E Antibody

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NSJ Bioreagents
Product Code: NSJ-R20306
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20306-50UG50ug£535.00
Quantity:
NSJ-R20306-100UG100ug£818.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM8
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant BRAF V600E antibody at -20oC.

Images

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Western blot analysis of cell lysates prepared from cell lines expressing endogenous mutant (V600E) BRAF or wild type (WT) protein with recombinant BRAF V600E antibody.
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IHC staining of formalin fixed and paraffin embedded melanoma tissue sections with recombinant BRAF V600E antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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IHC staining of formalin fixed and paraffin embedded thyroid carcinoma tissue with recombinant BRAF V600E antibody. Allele specific PCR validated to be positive for BRAF V600E. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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IHC staining of formalin fixed and paraffin embedded thyroid carcinoma tissue with recombinant BRAF V600E antibody. Allele specific PCR validated to be negative for BRAF V600E. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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ICC staining of formalin fixed and paraffin embedded human WiDr cells (400X, Positive) with recombinant BRAF V600E antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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ICC staining of formalin fixed and paraffin embedded human SW40 cells (400X, Negative) with recombinant BRAF V600E antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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Western blot analysis of cell lysates prepared from cell lines expressing endogenous mutant (V600E) BRAF or wild type (WT) protein with recombinant BRAF V600E antibody.
IHC staining of formalin fixed and paraffin embedded melanoma tissue sections with recombinant BRAF V600E antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC staining of formalin fixed and paraffin embedded thyroid carcinoma tissue with recombinant BRAF V600E antibody. Allele specific PCR validated to be positive for BRAF V600E. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC staining of formalin fixed and paraffin embedded thyroid carcinoma tissue with recombinant BRAF V600E antibody. Allele specific PCR validated to be negative for BRAF V600E. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
ICC staining of formalin fixed and paraffin embedded human WiDr cells (400X, Positive) with recombinant BRAF V600E antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
ICC staining of formalin fixed and paraffin embedded human SW40 cells (400X, Negative) with recombinant BRAF V600E antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 0.5-5 ug/ml,Immunocytochemistry: 2-10 ug/ml,Western Blot: 0.5-2 ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant BRAF V600E antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to the BRAF V600E mutant. No cross reactivity seen with wild type BRAF.
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to BRAF V600E mutant was used as the immunogen for the recombinant BRAF V600E antibody.
Limitation:
This recombinant BRAF V600E antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
Human
Uniprot #:
P15056