NKX2.2 Antibody

NSJ Bioreagents
Product Code: NSJ-V7226
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7226-20UG20 ug£264.00
Quantity:
NSJ-V7226-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: RMNK2-1
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant NKX2.2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human pancreas with recombinant NKX2.2 antibody (clone RMNK2-1). HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.~
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IHC testing of FFPE Ewings sarcoma with recombinant NKX2.2 antibody (clone RMNK2-1)

IHC testing of FFPE human pancreas with recombinant NKX2.2 antibody (clone RMNK2-1). HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.~
IHC testing of FFPE Ewings sarcoma with recombinant NKX2.2 antibody (clone RMNK2-1)

Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Titering of the recombinant NKX2.2 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
NKX2.2 acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter (By similarity). [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
4821
Immunogen:
A human partial recombinant protein was used as the immunogen for this recombinant NKX2.2 antibody.
Limitation:
This recombinant NKX2.2 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein A affinity chromatography
Species Reactivity :
Human