Neurofilament Antibody (-Heavy) / NF-H / NEFH

Western blot: 1-2ug/ml,Flow cytometry: 0.5-1ug/million cells,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT

The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the recombinant Neurofilament antibody to be titered for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Recombinant human protein (heavy subunit) was used as the immunogen for the recombinant Neurofilament antibody.

This recombinant Neurofilament antibody is available for research use only.

Cytoplasmic, membranous

Protein G affinity

Human, Mouse, Rat

P12036