Nucleophosmin Antibody / NPM1

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NSJ Bioreagents
Product Code: NSJ-V7981
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7981-20UG20 ug£264.00
Quantity:
NSJ-V7981-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rNPM1/1901
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant Nucleophosmin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Nucleophosmin antibody (clone rNPM1/1901); Red=isotype control, Blue= recombinant Nucleophosmin antibody.
2 / 5
Western blot testing of human K562 cell lysate with recombinant Nucleophosmin antibody. Predicted molecular weight ~38 kDa.
3 / 5
IHC staining of FFPE human skin with recombinant Nucleophosmin antibody (clone rNPM1/1901). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 5
IHC staining of FFPE human skin with recombinant Nucleophosmin antibody (clone rNPM1/1901). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
5 / 5
SDS-PAGE analysis of purified, BSA-free recombinant Nucleophosmin antibody (clone rNPM1/1901) as confirmation of integrity and purity.
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Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Nucleophosmin antibody (clone rNPM1/1901); Red=isotype control, Blue= recombinant Nucleophosmin antibody.
Western blot testing of human K562 cell lysate with recombinant Nucleophosmin antibody. Predicted molecular weight ~38 kDa.
IHC staining of FFPE human skin with recombinant Nucleophosmin antibody (clone rNPM1/1901). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human skin with recombinant Nucleophosmin antibody (clone rNPM1/1901). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Nucleophosmin antibody (clone rNPM1/1901) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml (not suitable for frozen tissue sample testing),Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells in 0.1ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Nucleophosmin (NPM) is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant human partial protein (amion acids 185-287) was used as the immunogen for this recombinant Nucleophosmin antibody.
Limitation:
This recombinant Nucleophosmin antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P06748