Parathyroid Hormone Antibody / N Terminal

Immunohistochemistry (FFPE; not suitable for frozen tissues): 0.5-1ug/ml for 30 min at RT

Optimal dilution of the recombinant Parathyroid Hormone antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

PTH/Parathyroid hormone is a hormone produced by the parathyroid gland that regulates the concentration of calcium and phosphorus in extracellular fluid. This hormone elevates blood Ca2+ levels by dissolving the salts in bone and preventing their renal excretion. It is produced in the parathyroid gland as an 84 amino acid single chain polypeptide. It can also be secreted as N-terminal truncated fragments or C-terminal fragments after intracellular degradation, as in case of hypercalcemia. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH); also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

A synthetic peptide from the N-terminal region of the human protein was used as the immunogen for the recombinant Parathyroid Hormone antibody.

This recombinant Parathyroid Hormone antibody is available for research use only.

Cytoplasmic and secreted

Protein G affinity chromatography

Human

P01270