TG Antibody / Thyroglobulin

Flow cytometry: 0.5-1ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT

Optimal dilution of the recombinant Thyroglobulin antibody should be determined by the researcher.

mAb rTGB/4744 reacts with a partially defined epitope of human thyroglobulin. This epitope is different form the epitope recognized by mAb 6E1. Thyroglobulin is a 660kDa dimeric pre-protein with mutiple glycosylation sites. It is produced by and processed within the thyroid gland to produce the hormone thyroxine and triiodothyronine. Prior to forming dimers, thyroglobulin monomers undergo conformational maturation in the endoplasmic reticulation. The vast majority of follicular carcinomas of the thyroid will give positive immunoreactivity for anti-thyroglobulin even though sometimes only focally. Poorly differentiated carcinomas of the thyroid are frequently anti-thyroglobulin negative. Adenocarcinomas of other-than-thyroid origin do not react with this antibody. This antibody is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of thyroglobulin in metastatic lesions establishes the thyroid origin of tumor. Anti-thyroglobulin, combined with anti-calcitonin, can identify medullary carcinomas of the thyroid. Furthermore, anti-thyroglobulin, combined with anti-TTF1, can be a reliable marker to differentiate between primary thyroid and lung neoplasms.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Human thyroid follicular cells were used as the immunogen for the recombinant Thyroglobulin antibody.

This recombinant Thyroglobulin antibody is available for research use only.

Cytoplasmic, secreted

Protein G affinity chromatography

Human

P01266