p40 / deltaNp63 Antibody

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NSJ Bioreagents
Product Code: NSJ-V8627
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8627-20UG20 ug£264.00
Quantity:
NSJ-V8627-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rTP40/3690
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant p40 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human kidney with recombinant p40 antibody (clone rTP40/3690). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human skin with recombinant p40 antibody (clone rTP40/3690). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Flow cytometry testing of PFA-fixed human HeLa cells with recombinant p40 antibody (clone rTP40/3690); Red=isotype control, Blue= recombinant p40 antibody.
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IHC staining of FFPE human kidney with recombinant p40 antibody (clone rTP40/3690). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human skin with recombinant p40 antibody (clone rTP40/3690). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of PFA-fixed human HeLa cells with recombinant p40 antibody (clone rTP40/3690); Red=isotype control, Blue= recombinant p40 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the recombinant p40 antibody should be determined by the researcher.
Description:
p63 consists of two major isoforms-TAp63 and delta-Np63. These isoforms differ in the structure of the N-terminal domains. The TAp63 isoform (identified by anti-p63 antibody) contains a transactivation-competent TA domain with homology to p53, which regulates the expression of the growth-inhibitory genes. In contrast, DNp63 isoform (identified by anti-p40 antibody) contains an alternative transcriptionally-inactive delta-N domain, which antagonizes the activity of TAp63 and p53. P40/3980R recognizes exclusively delta-Np63 but not TAp63. p40 is a squamous cell carcinoma specific antibody. It reacts with the vast majority of cases of squamous cell carcinomas of various origins, but not with adenocarcinomas. It is particularly useful in differentiating lung squamous cell carcinoma from lung poorly differentiated adenocarcinoma. p40 antibody can also be used as an alternative basal cell/myoepithelial cell marker, which has similar sensitivity and specificity as that of p63 antibody. Therefore, p40 antibody may also be used as an alternative immunohistochemical marker for determining prostate adenocarcinoma vs. benign prostate glands and for determining breast intraductal carcinoma vs. invasive breast ductal carcinoma.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A synthetic peptide from the N-terminal of human p40 protein was used as the immunogen for the recombinant p40 antibody.
Limitation:
This recombinant p40 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
Q9H3D4