Thymidine Phosphorylase Antibody / PD-ECGF

Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT,Western blot: 1-2ug/ml

Optimal dilution of the recombinant PD-ECGF antibody should be determined by the researcher.

Recognizes a protein (amino acid 482) of 55kDa (in vivo 110kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5-deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Recombinant full-length human protein was used as the immunogen for the recombinant PD-ECGF antibody.

This recombinant PD-ECGF antibody is available for research use only.

Cytoplasmic, nuclear

Protein G affinity chromatography

Human

P19971