PGP9.5 / UchL1 Antibody

NSJ Bioreagents
Product Code: NSJ-V8654
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8654-20UG20 ug£264.00
Quantity:
NSJ-V8654-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rUCHL1/4557
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant PGP9.5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 4
IHC staining of FFPE human cerebellum with recombinant PGP9.5 antibody (clone rUCHL1/4557). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human cerebellum with recombinant PGP9.5 antibody (clone rUCHL1/4557). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human brain lysate with recombinant PGP9.5 antibody. Predicted molecular weight ~25 kDa.
4 / 4
SDS-PAGE analysis of purified, BSA-free recombinant PGP9.5 antibody (clone rUCHL1/4557) as confirmation of integrity and purity.

IHC staining of FFPE human cerebellum with recombinant PGP9.5 antibody (clone rUCHL1/4557). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cerebellum with recombinant PGP9.5 antibody (clone rUCHL1/4557). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human brain lysate with recombinant PGP9.5 antibody. Predicted molecular weight ~25 kDa.
SDS-PAGE analysis of purified, BSA-free recombinant PGP9.5 antibody (clone rUCHL1/4557) as confirmation of integrity and purity.

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the recombinant PGP9.5 antibody should be determined by the researcher.
Description:
This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinsons disease.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human protein was used as the immunogen for the recombinant PGP9.5 antibody.
Limitation:
This recombinant PGP9.5 antibody is available for research use only.
Localization:
Cytoplasmic, ER membrane
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P09936