S100A4 Antibody / FSP1

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NSJ Bioreagents
Product Code: NSJ-V3434BTN
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3434BTN-500ul500 ul£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: S100A4/1481
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the FSP1 antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).

Images

1 / 4
IHC testing of FFPE human placenta with biotinylated FSP1 antibody (clone S100A4/1481). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 4
IHC testing of FFPE human placenta with biotinylated FSP1 antibody (clone S100A4/1481). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using S100A4/FSP1 antibody (clone S100A4/1481). These results demonstrate the foremost specificity of the S100A4/1481 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
4 / 4
SDS-PAGE analysis of purified, BSA-free FSP1 antibody as confirmation of integrity and purity.
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IHC testing of FFPE human placenta with biotinylated FSP1 antibody (clone S100A4/1481). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC testing of FFPE human placenta with biotinylated FSP1 antibody (clone S100A4/1481). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using S100A4/FSP1 antibody (clone S100A4/1481). These results demonstrate the foremost specificity of the S100A4/1481 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free FSP1 antibody as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Flow cytometry: 5ul/million cells,Western blot: 2-4ug/ml,Immunofluorescence: 2-4ug/ml at RT,Immunohistochemistry (FFPE): 2-4ug/ml at RT
Application Note:
Optimal dilution of the FSP1 antibody should be determined by the researcher.
Description:
S100A4/FSP1 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4/FSP1 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 4 integrin, and liprin b1. It is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.
Format :
Biotin Conjugate
Formulation :
0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 1-200 from the human protein was used as the immunogen for the FSP1 antibody.
Limitation:
This FSP1 antibody is available for research use only.
Localization:
Cytoplasmic and nuclear
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Mouse
Uniprot #:
P26447